A new set of multiplexes PCR suitable for genomic studies in Saudi sheep breeds

Abstract. A total of 36 microsatellite markers (Bl42, BM1861, BM2504, BM864, BMC1222, BMS1620, BMS1948, BMS522, BMS975, BRN, CSRD2111, CSRD263, CSSM008, CSSM015, CSSM25, CSSM41, CSSM43, CSSM66, FAS, HMH1R, ILSTS011, LSCV29, MAF33, MCM1, MCM136, MCM42, MCMA26, OARCP23, OARCP49, OARCP134, OARKP6, OCAM, RBP3, TGLA53, TGLA322, URB48) were used to investigate the possibility of being amplified set of multiplexes PCR. The markers are located in 20 different autosomic ovine chromosomes. The results showed that the 36 markers used in this study could be amplified together in nine different multiplexes. Each three multiplexes labeled by different fluorescent color (FAM, VIC and NED) gathered to be loaded in the same lane. The three multi-loads contained 11, 13 and 12 markers, respectively. 91.67% of the 36 markers have more than 4 alleles. These results are good indicator of the high variability of the selected markers in our population.